Rapid Categorization of PathogenicEscherichia coliby Multiplex PCR

Rapid diagnosis of leptospirosis by multiplex PCR.

BACKGROUND Traditionally, the most common diagnostic approach used for diagnosing leptospirosis was the demonstration of immune-seroconversion in acute and convalescent patient serum samples. Recently, a variety of molecular techniques, including conventional and real-time polymerase chain reaction (PCR), have been developed for the specific detection of pathogenic bacteria from the genus Lepto...

Rapid detection of carbapenemase genes by multiplex real-time PCR.

OBJECTIVES To develop a single multiplex real-time PCR assay to detect six different genetic types of carbapenemases already identified in Enterobacteriaceae (KPC, GES, NDM, IMP, VIM and OXA-48). METHODS A total of 58 bacterial isolates were tested. Thirty were previously characterized as resistant to carbapenems and documented by PCR and sequencing analysis to carry the following genes: bla(...

Rapid detection of methicillin-resistant staphylococci by multiplex PCR.

A multiplex PCR was developed to detect the coagulase gene (coa; pathognomic of Staphylococcus aureus) and the mecA gene (characteristically encoding for methicillin resistance in staphylococci) in a single, rapid test. Suitable primers for the gene targets and an internal, amplification control were incorporated into a multiplex PCR assay, which was then optimized on a capillary air thermal cy...

Rapid and Concurrent Detection of Listeria Species by Multiplex Pcr

Listeria sps are ubiquitous in nature. Infection due to L. monocytogenes causes illness in animals and humans worldwide. Aim of the present study was to standardize a multiplex PCR for the identification and differentiation of important Listeria species, in particular, Listeria monocytogenes and to detect toxigenic potential of L. monocytogenes. Employing primers for truncated regions of seven ...

Multiplex Touchdown PCR for Rapid Typing of the Opportunistic Pathogen